NR AFKS
AU Hochstrasser,D.F.; Frutiger,S.; Wilkins,M.R.; Hughes,G.; Sanchez,J.C.
TI Elevation of apolipoprotein E in the CSF of cattle affected by BSE
QU FEBS Letters 1997 Oct 20; 416(2): 161-3
PT journal article
AB The cerebrospinal fluid (CSF) of patients suffering from Creutzfeldt-Jakob disease (CJD) display two unique polypeptide chains by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). In the absence of a well-defined ante-mortem diagnostic test for bovine spongiform encephalopathy (BSE), spinal fluid samples of eight normal cows and eight cows known to carry BSE by post-mortem histological analysis were investigated to verify if equivalent polypeptides were present. Proteins with similar migration to human CJD polypeptides were not detected. But surprisingly, a cluster of polypeptide spots that was faint or not detected in normal bovine CSF samples was found to be elevated or massively increased in BSE CSF samples (more than 10-fold increase). These elevated polypeptide chains were identified as apolipoprotein E.
MH Amino Acid Sequence; Animal; Apolipoproteins E/*cerebrospinal fluid/chemistry; Cattle; Creutzfeldt-Jakob Syndrome/*cerebrospinal fluid; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Encephalopathy, Bovine Spongiform/*cerebrospinal fluid; Human; Molecular Sequence Data; Peptide Fragments/chemistry; Reference Values; Reproducibility of Results; Species Specificity
AD Clinical Chemistry Laboratory, Geneva University Hospital (HUG), Switzerland.
SP englisch
PO Niederlande