NR AFOC
AU Horiuchi,M.; Ishiguro,N.; Nagasawa,H.; Toyoda,Y.; Shinagawa,M.
TI Alternative usage of exon 1 of bovine PrP mRNA
QU Biochemical and Biophysical Research Communications 1997 Apr 28; 233(3): 650-4
PT journal article
AB Here we report two types of bovine prion protein (PrP) mRNA that possessed different lengths of the 5'-untranslated region and were expressed in various bovine tissues. The two mRNA species were transcribed from identical positions but differed in the usage of the splice site for exon 1/intron. One mRNA possessed exon 1 consisting of 53 nucleotides and the other possessed exon 1 consisting of 168 nucleotides. Usage of exons 2 and 3 was identical for the two mRNA species. The two mRNA species were detected in all but spleen tissue; the mRNA possessing 168-nt exon 1 was not detected in bovine spleen. This is the first report on the tissue-specific alternative splicing of PrPc mRNA in any other species. Only a low level of PrPc appeared to be present in bovine spleen. These results suggested the possibility that the mRNA possessing 53-nt exon 1 was inefficiently translated into Prp; however, in vitro translation analysis showed no marked difference in translational efficiency between the two mRNA species.
MH Alternative Splicing; Animal; Base Sequence; Cattle; Cloning, Molecular; DNA Primers/genetics; DNA, Complementary/genetics; Encephalopathy, Bovine Spongiform/genetics/metabolism; *Exons; Molecular Sequence Data; Prions/*genetics; RNA, Messenger/genetics/metabolism; Sheep; Support, Non-U.S. Gov't; Tissue Distribution; Translation, Genetic
AD Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Japan.
SP englisch
PO USA