NR AFOO
AU Hornemann,S.; Glockshuber,R.
TI Autonomous and reversible folding of a soluble amino-terminally truncated segment of the mouse prion protein
QU Journal of Molecular Biology 1996 Sep 6; 261(5): 614-9
PT journal article
AB Prion diseases are assumed to be caused by the infectious isoform, PrPsc, of a single cellular surface protein, PrPc. PrPsc is an insoluble form of PrPc and is believed to possess a different three-dimensional fold. It may propagate by causing PrPc to adopt its own infectious conformation by an unknown mechanism. Studies on folding and thermodynamic stability of prion proteins are essential for understanding the processes underlying the conversion from PrPc to PrPsc, but have so far been hampered by the low solubility of prion proteins in the absence of detergents. Here, we show that the amino-terminally truncated segment of mouse PrP comprising residues 121 to 231 is an autonomous folding unit. It consists predominantly of alpha-helical secondary structure and is soluble at high concentrations up to 1 mM in distilled water. PrP(121-231) undergoes a cooperative and completely reversible unfolding/refolding transition in the presence of guanidinium chloride with a free energy of folding of -22 kJ/mol at pH 7. The intrinsic stability of segment 121-231 is not in accordance with present models of the structure of PrPc and PrPsc PrP(121-231) may represent the only part of PrPc with defined three-dimensional structure.
IN Die Autoren behaupten, ein den Aminosäuren 121-231 des Prionproteins entsprechendes Peptid stelle eine autonome Faltungseinheit dar. Es bildet bei Konzentrationen von bis zu 1 mM in destilliertem Wasser überwiegend Alphahelices, welche sich in Gegenwart von Guanidiniumchlorid reversibel auflösen. Dabei wurde eine freie Faltungsenergie von -22 kJ/mol bei pH 7 gemessen. Die Tatsache, dass die Struktur ihres Fragmentes Modellen der Prionproteinfaltung widerspricht, ist den Autoren bewußt, macht sie aber nicht sehr nachdenklich.
ZR 34
MH Animal; Base Sequence; Circular Dichroism; Escherichia coli/genetics; Glycosylation; Mice; Models, Molecular; Molecular Sequence Data; Peptide Fragments/chemistry; PrPc Proteins/chemistry/genetics/metabolism; Prions/*chemistry/genetics/metabolism; Protein Conformation; *Protein Folding; Recombinant Proteins/biosynthesis/chemistry/genetics; Solubility; Support, Non-U.S. Gov't; Thermodynamics; Ultraviolet Rays
AD Institut für Molekularbiologie und Biophysik Eidgenössische Technische Hochschule Hönggerberg, Zürich, Switzerland.
SP englisch
PO England