NR AFUY
AU Iqbal,K.; Somerville,R.A.; Thompson,C.H.; Wisniewski,H.M.
TI Brain glutamate decarboxylase and cholinergic enzyme activities in scrapie
QU Journal of the Neurological Sciences 1985 Mar; 67(3): 345-50
PT journal article
AB C57BL/6J mice, age 6-8 weeks were inoculated intracerebrally with brain homogenate from mice previously infected with the 139A strain of scrapie; control mice were identically treated with brain homogenate from non-infected normal mice. The activities of choline acetyltransferase (CAT), acetyl cholinesterase (AChE), and glutamic acid decarboxylase (GAD) were determined in the forebrain and hindbrain of these animals after 67, 126 and 151 days post-inoculation. There were no significant differences in the activities of CAT and GAD between scrapie and control mice at early, middle or late stages of the disease in the scrapie-infected animals; there was an about 20% decline in AChE activity in the scrapie brain.
IN 6-8 Wochen alte C57BL/6J-Mäuse wurden intracerebral mit Hirnhomogenat von ihrerseits mit dem Scrapiestamm 139A infizierten Mäusen inokuliert. Kontrollmäusen wurde ebenfalls intracerebral Hirnhomogenat gesunder Mäuse inokuliert. Die Aktivitäten der Cholinacetyltransferase und der Glutamatdecarboxylase waren 67, 126 und 151 Tage nach der Infektion in Vor- und Hinterhirn unverändert. Bei der Acetylcholinesterase wurde jedoch eine Abnahme der Aktivität um etwa 20% beobachtet.
MH Acetylcholinesterase/*metabolism; Alzheimer Disease/enzymology; Animal; Brain Diseases/enzymology; Choline O-Acetyltransferase/*metabolism; Comparative Study; Dementia/enzymology; Female; Glutamate Decarboxylase/*metabolism; Human; Mice; Mice, Inbred C57BL; Neurofibrils; Parkinson Disease/enzymology; Scrapie/*enzymology; Sheep; Support, U.S. Gov't, P.H.S.
SP englisch
PO Niederlande