NR AGEL
AU Kanemaru,K.; Hasegawa,M.; Shimada,H.; Ihara,Y.
TI The presence of a novel protein in calf serum that recognizes beta amyloid in the formalin-fixed section
QU American Journal of Pathology 1990 Sep; 137(3): 677-87
PT journal article
AB Here we report on a monoclonal antibody, H6-33, that labels various beta-amyloid plaques, including diffuse plaques in the formalin-fixed, paraffin-embedded section from the brain affected with Alzheimer's disease (AD), without formic acid pretreatment. H6-33 also labels some neurofibrillary tangles and all kuru plaques in Gerstmann-Sträussler-Scheinker disease. In sharp contrast, H6-33 did not stain beta amyloid in the leptomeningeal vessel. For specific staining, H6-33 required the presence of fetal calf serum and it was necessary for beta amyloid to be formalin fixed. These results suggest that a novel protein in the calf serum, CSX, binds formalin-fixed beta amyloid, followed by H6-33 binding. The detection of beta amyloid by CSX was nullified by formic acid pretreatment of the tissue section. In accordance with this, CSX reacted only with a polymer form of synthetic beta peptide after fixation, but not with native beta-protein or beta-peptide monomer. These observations strongly suggest that 1) meningovascular beta amyloid should have a beta-pleated sheet structure somewhat dissimilar to that of beta-amyloid cores; and 2) most, if not all, of beta-protein immunoreactivities of diffuse plaques in AD sections are presumably derived from small amounts of amyloid fibrils scattered in the normal-looking neurohil.
MH Alzheimer Disease/*pathology; Amyloid/*analysis/immunology; Animal; Antibodies, Monoclonal/diagnostic use; Blood Proteins/*immunology; Brain/pathology; Cattle; Enzyme-Linked Immunosorbent Assay; Human; Immunoassay; Immunoenzyme Techniques; Staining and Labeling; Support, Non-U.S. Gov't
AD Second Laboratory of Clinical Physiology, Tokyo Metropolitan Institute of Gerontology, Japan.
SP englisch
PO USA