NR AGFC
AU Kascsak,R.J.; Rubenstein,R.; Merz,P.A.; Carp,R.I.; Robakis,N.K.; Wisniewski,H.M.; Diringer,H.
TI Immunological comparison of scrapie-associated fibrils isolated from animals infected with four different scrapie strains
QU Journal of Virology 1986 Sep; 59(3): 676-83
PT journal article
AB Scrapie-associated fibrils (SAFs) are abnormal filamentous structures that are uniquely associated with unconventional slow virus diseases. The antigenic relationships of SAFs from animals infected with four biologically distinct scrapie strains were investigated by using antisera raised to purified SAF proteins. Rabbit antisera were raised to SAFs isolated from mice infected with the ME7 scrapie strain and to SAFs isolated from hamsters infected with the 263K scrapie strain. A strong antigenic relationship was shown among SAF proteins (PrPs) isolated from all scrapie-infected animals (ME7, 139A, and 87V in mice and 263K in hamsters), and this relationship was demonstrable regardless of which antiserum was used. SAF proteins were antigenically distinct from those of paired helical filaments or amyloid isolated from patients with Alzheimer disease. Distinct Western blot profiles were demonstrated for SAFs isolated from animals infected with each scrapie strain. Differences seen among SAFs were independent, at least in part, of host species or genotype, implying that certain specific structural and molecular properties of SAFs are mediated by the strain of scrapie agent.
IN Von Kaninchen produzierte Antikörper gegen scrapieassoziierte Fibrillen aus mit ME7-Mausscrapie infizierten Mäusen bzw. mit 263K-Hamsterscrapie infizierten Hamstern markierten die Proteine aus scrapieassoziierten Fibrillen mit den Erregerstämmen ME7, 139A und 87V infizierter Mäuse sowie mit dem Stamm 263K infizierten Hamstern. Die Bandenmuster der aus scrapieassoziierten Fibrillen herausgelösten Peptide im Westernblot sind je nach dem verwendeten Erregerstamm unterschiedlich.
MH Alzheimer Disease/metabolism; Amyloid/analysis/immunology; Animal; Comparative Study; Epitopes; Hamsters; Immune Sera; Mice; Mice, Inbred C57BL; Microscopy, Electron; Nerve Tissue Proteins/analysis/*immunology/isolation & purification; PrP 27-30 Protein; Prions/*physiology; Scrapie/metabolism/*microbiology; Support, U.S. Gov't, P.H.S.
SP englisch
PO USA
OR Prion-Krankheiten 5