NR AGKI
AU Kimberlin,R.H.; Hall,S.M.; Walker,C.A.
TI Pathogenesis of mouse scrapie. Evidence for direct neural spread of infection to the CNS after injection of sciatic nerve.
QU Journal of the Neurological Sciences 1983 Oct-Nov; 61(3): 315-25
PT journal article
AB Previous studies of peripherally injected mouse scrapie suggested that invasion of the CNS occurs initially in mid-thoracic cord by neural spread of infection from spleen and other visceral sites of extraneural replication. We now show that infection of the left sciatic nerve leads to direct spread of infection to brain (at a rate of approximately 1.0-2.0 mm/day), bypassing the need for extraneural replication and thus producing shorter incubation periods. However, the efficiency of intraneural infection is low. It can be increased by crush injury or by the injection of lysophosphatidyl choline, both of which temporarily increase the surface area of axolemma exposed to inoculum. Once infection is established, agent seems to spread throughout the nervous system but, at the clinical stage of disease, the titres in the PNS are much lower than in the CNS.
IN Nach Injektionen von Scrapie-Agens in den linken Ischiasnerv, soll die Infektiosität um in Mäusen 1-2 mm pro Tag in Richtung Gehirn gewandert sein und durch Umgehung extraneuraler Amplifikation zu kürzeren Inkubationszeiten geführt haben. Die Effizienz dieser intraneuralen Infektionen war gering, konnte jedoch durch Quetschverletzungen oder Lysophosphatidylcholin-Injektionen bzw. durch die dadurch ausgelöste zeitweise Zunahme der dem Inokulum ausgesetzten Axolemmaoberfläche gesteigert werden.
MH Animal; Brain/*microbiology; Female; Mice; Mice, Inbred Strains; Prions/pathogenicity; Sciatic Nerve/*microbiology; Scrapie/*microbiology; Sheep; Spinal Cord/*microbiology; Spleen/microbiology; Virus Replication
SP englisch
PO Niederlande