NR AGKQ
AU Kimberlin,R.H.; Walker,C.A.
TI Pathogenesis of mouse scrapie: dynamics of agent replication in spleen, spinal cord and brain after infection by different routes.
QU Journal of Comparative Pathology 1979 Oct; 89(4): 551-62
PT journal article
AB Groups of Compton white mice were infected with different doses of the Chandler (139A) strain of agent by one of the following routes of injection; intracerebral, intraperitoneal, intravenous, subcutaneous in the "scruff" of the neck or under the footpad. At weekly intervals, 3 mice were taken from each group and infectivity assays were performed on pools of spleen, brain and spinal cord to study the dynamics of agent replication during the early stages of incubation. All peripheral routes of infection gave a similar pattern in that agent replication started early in spleen and reached a plateau concentration of agent before replication occurred in spinal cord. Replication in spinal cord occurred after 25 to 42 per cent of the incubation period had elapsed and always preceded the onset of replication in brain (41 to 55 per cent of incubation period). This sequence is consistent with the spread of agent from spleen to spinal cord and thence to brain, possibly along neural pathways. Agent replication in brain started much sooner (13 to 19 per cent of incubation period) with the i.c. route of infection but replication in spinal cord followed the pattern found with peripheral routes of infection and occurred after 24 to 34 per cent of the incubation had elapsed.
MH Animal; Brain/*microbiology; Comparative Study; Female; Injections; Injections, Intraperitoneal; Injections, Intravenous; Injections, Subcutaneous; *Mice; Prions/*growth & development/isolation & purification; Rodent Diseases/*microbiology; Scrapie/*microbiology; Sheep; Spinal Cord/*microbiology; Spleen/*microbiology; Virus Replication
AD Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Nr. Newbury, Berks RG16 0NN, U.K.
SP englisch
PO England
OR Prion-Krankheiten 5