NR AGNG
AU Kitamoto,T.; Nakamura,K.; Nakao,K.; Shibuya,S.; Shin,R.W.; Gondo,Y.; Katsuki,M.; Tateishi,J.
TI Humanized prion protein knock-in by Cre-induced site-specific recombination in the mouse
QU Biochemical and Biophysical Research Communications 1996 May 24; 222(3): 742-7
PT journal article
AB To establish humanized mice with a knock-in (gene replacement) technique, we constructed a targeting vector which consists of the human prion protein and the loxP sequences. The introduced human prion protein with the loxP system in the embryonic stem cells was transmitted through the mouse germ line. Transient expression of Cre recombinase in the fertilized eggs resulted in the prion protein humanized mice. The Cre-loxP-mediated gene replacement is a simple and efficient method which is generally applicable to make humanized animal models.
IN Mit Hilfe der Technik des The Cre-loxP-vermittelten Genersetzens erzeugen die Autoren Mäuse mit menschlichen Prionproteinen.
ZR 21
MH Animal; Base Sequence; DNA Nucleotidyltransferases/metabolism; DNA Primers/chemistry; Genetic Engineering/*methods; Human; Mice; Mice, Transgenic; Molecular Sequence Data; Prions/*genetics; Recombination, Genetic; Support, Non-U.S. Gov't
AD Department of Neurological Science, Tohoku University School of Medicine, Sendai, Japan.
SP englisch
PO USA