NR AGOA
AU Kitamoto,T.; Hikita,K.; Tashima,T.; Tateishi,J.; Sato,Y.
TI Scrapie-associated fibrils (SAF) purification method yields amyloid proteins from systemic and cerebral amyloidosis
QU Bioscience Reports 1986 May; 6(5): 459-65
PT journal article
AB We identified fibrils from non-transmissible systemic and cerebral amyloidosis using the purification method of scrapie-associated fibrils (SAF). The fibrils possessed the same nature of congophilia, filamentous structures and molecular weights as amyloid fibrils, and were resistant to Proteinase K digestion. This SAF method makes for a rapid extraction from amyloid-laden tissues. The method, therefore, may purify nontransmissible amyloids alone or together with SAF proteins.
MH Amyloid/*isolation & purification; Amyloidosis/*metabolism; Brain Diseases/*metabolism; Endopeptidase K; Endopeptidases; Human; Microscopy, Electron; Nerve Tissue Proteins/*isolation & purification; PrP 27-30 Protein; Support, Non-U.S. Gov't
SP englisch
PO England