NR AGOT
AU Klonowski,W.
TI Non-equilibrium proteins
QU Computers and Chemistry 2001 Jul; 25(4): 349-68
PT journal article; review; review, tutorial
AB There exist no methodical studies concerning non-equilibrium systems in cellular biology. This paper is an attempt to partially fill this shortcoming. We have undertaken an extensive data-mining operation in the existing scientific literature to find scattered information about non-equilibrium subcellular systems, in particular concerning fast proteins, i.e. those with short turnover half-time. We have advanced the hypothesis that functionality in fast proteins emerges as a consequence of their intrinsic physical instability that arises due to conformational strains resulting from co-translational folding (the interdependence between chain elongation and chain folding during biosynthesis on ribosomes). Such intrinsic physical instability, a kind of conformon (Klonowski-Klonowska conformon, according to Ji, (Molecular Theories of Cell Life and Death, Rutgers University Press, New Brunswick, 1991)) is probably the most important feature determining functionality and timing in these proteins. If our hypothesis is true, the turnover half-time of fast proteins should be positively correlated with their molecular weight, and some experimental results (Ames et al., J. Neurochem. 35 (1980) 131) indeed demonstrated such a correlation. Once the native structure (and function) of a fast protein macromolecule is lost, it may not be recovered - denaturation of such proteins will always be irreversible; therefore, we searched for information on irreversible denaturation. Only simulation and modeling of protein co-translational folding may answer the questions concerning fast proteins (Ruggiero and Sacile, Med. Biol. Eng. Comp. 37 (Suppl. 1) (1999) 363). Non-equilibrium structures may also be built up of protein subunits, even if each one taken by itself is in thermodynamic equilibrium (oligomeric proteins; sub-cellular sol-gel dissipative network structures).
ZR 129
MH Amino Acid Sequence; Animal; Bacterial Proteins/metabolism; Cattle; Comparative Study; Glutamic Acid/chemistry; Human; Macromolecular Systems; Models, Molecular; Models, Theoretical; Nitric Oxide/metabolism; Prions/metabolism; Proline/chemistry; Protein Conformation; Protein Denaturation; Protein Engineering; Protein Folding; Proteins/*chemistry/metabolism; Rats; Serine/chemistry; Structure-Activity Relationship; Threonine/chemistry
AD Laboratory of Biosignal Analysis Fundamental, Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Warsaw. wklon@hrabia.ibib.waw.pl
SP englisch
PO England