NR AGQA
AU Kochneva-Pervukhova,N.V.; Poznyakovski,A.I.; Smirnov,V.N.; Ter-Avanesyan,M.D.
TI C-terminal truncation of the Sup35 protein increases the frequency of de novo generation of a prion-based [PSI+] determinant in Saccharomyces cerevisiae
QU Current Genetics 1998 Aug; 34(2): 146-51
PT journal article
AB The yeast non-Mendelian [PSI+] determinant is presumed to be the manifestation of the aggregated prion-like state of the Sup35 protein. Plasmid-mediated amplification of the SUP35 gene greatly increases the frequency of Sup35p transition to this prion-like state. Here we show that the 3'-deletions of plasmid SUP35, leading to the C-terminal truncation of Sup35p, further increase the frequency of [PSI+] induction despite a marked decrease in Sup35p expression levels. The data suggest that the presence of Sup35p N-terminal proteolytic fragments can cause [PSI+] appearance in wild-type yeast cells.
MH Alleles; Fungal Proteins/*genetics; Gene Amplification; Gene Expression; Genes, Fungal; Plasmids/genetics; Prions/*genetics; Saccharomyces cerevisiae/*genetics/*metabolism; Sequence Deletion; Support, Non-U.S. Gov't; Transformation, Genetic
AD Institute of Experimental Cardiology, Cardiology Research Center, 121552 Moscow, Russia. vita@excard.msk.su
SP englisch
PO USA