NR AGYV
AU Ladogana,A.; Casaccia,P.; Ingrosso,L.; Cibati,M.; Salvatore,M.; Xi,Y.G.; Masullo,C.; Pocchiari,M.
TI Sulphate polyanions prolong the incubation period of scrapie-infected hamsters
QU Journal of General Virology 1992 Mar; 73(3): 661-5
PT journal article
AB The effect of the organic sulphated polyanions, pentosan sulphate (SP54), dextran sulphate 500 (DS500) and suramin, have been tested on golden Syrian hamsters infected with the 263K strain of scrapie by the intraperitoneal (i.p.) or the intracerebral route. SP54 had the greatest effect in prolonging the incubation period of the disease when administered within 2 h of the i.p. inoculum. The same amount of SP54 given 24 h after scrapie inoculation had a potent effect in some animals and no effect in others. This result suggests that SP54 inhibits the uptake of the scrapie agent into the nerve endings and/or carrier cells at the site of the inoculum, i.e. the peritoneum, and that this event occurs in about 24 h. DS500 had a similar although less potent effect (22.4 days delay during the incubation period) than SP54 (54.4 days) when administered within 2 h of scrapie injection by the i.p. route, and suramin had only a minimal effect (10 days). This study suggests that treatment of scrapie and related spongiform encephalopathies of animals and man is possible only before the agent has reached the clinical target areas of the brain.
IN Bei intraperitoneal oder intracerebral mit dem Scrapiestamm 263K infizierten Hamstern verlängerte sich die Inkubationszeit, wenn sie mit den organischen sulphatierten Polyanionen Pentosansulphat 54, Dextransulphat 500 oder Suramin behandelt wurden. Der Effekt war am stärksten, wenn die Behandlung binnen 2 Stunden nach der Inokulation erfolgte. Danach war die Behandlung bei einigen Tieren effektiv, bei anderen jedoch nicht. Die beobachteten Verlängerungen der Inkubationszeiten waren bei Pentosansulphat 54 mit 54,4 Tagen deutlich größer als bei Dextransulphat 500 mit 22,4 Tagen und Suramin mit nur 10 Tagen.
MH Animal; Dextran Sulfate/*therapeutic use; Dose-Response Relationship, Drug; Hamsters; Mesocricetus/microbiology; Pentosan Sulfuric Polyester/*therapeutic use; Prions/*pathogenicity; Scrapie/*drug therapy; Sheep; Support, Non-U.S. Gov't; Suramin/*therapeutic use; Time Factors
AD Institute of General Pathology, Catholic University, Rome, Italy.
SP englisch
PO England