NR AHDP
AU Lee,K.H.; Harrington,M.G.
TI The assay development of a molecular marker for transmissible spongiform encephalopathies
QU Electrophoresis 1997 Mar-Apr; 18(3-4): 502-6
PT journal article
AB The transmissible spongiform encephalopathies are a group of neurodegenerative diseases which include Creutzfeldt-Jakob disease (CJD) in humans, scrapie in sheep, and bovine spongiform encephalopathy (BSE) in cattle. Two-dimensional electrophoresis of proteins was previously used to identify two marker proteins, 130/131, which are selectively present in the cerebrospinal fluid (CSF) of patients with CJD and not in patients with other dementias. The recent characterization of these proteins by amino acid sequencing has identified them as members of the 14-3-3 family of proteins. Polyclonal antibodies against 14-3-3 (all isoforms), 14-3-3 gamma, 14-3-3 beta, and 14-3-3 theta are immunoreactive with a 30 kDa marker band from CJD CSF. Silver staining of two-dimensional electrophoresis separated BSE CSF proteins does not identify a similar marker. However, 14-3-3 immunoreactivity is found in cattle CSF when these proteins are blotted to polyvinylidene difluoride but not when blotted to nitrocellulose.
IN Polyklonale Antikörper gegen die gamma-, die beta- und die theta-Isoform der Familie der 14-3-3-Proteine markieren eine 30 kD-Bande in der Rückenmarksflüssigkeit von Creutzfeldt-Jakob-Patienten. Nach Silberfärbung zweidimensional getrennter Proteine aus der Rückenmarksflüssigkeit von BSE-Rindern konnte kein vergleichbarer Marker gefunden werden. Antikörper gegen 14-3-3-Proteine markierten Banden von Rinderproteinen, wenn auf Polyvinylidendifluorid, nicht jedoch, wenn auf Nitrozellulose geblottet wurde.
ZR 11
MH Animal; Biological Markers/analysis; Cattle; *Electrophoresis, Gel, Two-Dimensional; Human; Prion Diseases/*enzymology; Proteins/*analysis; Support, U.S. Gov't, P.H.S.
AD Beckman Institute, California Institute of Technology, Pasadena 91125, USA
SP englisch
PO Deutschland