NR AHGA
AU Li,L.; Lindquist,S.L.
TI Creating a protein-based element of inheritance
QU Science 2000 Jan 28; 287(5453): 661-4
KI Science. 2000 Jan 28;287(5453):562-3. PMID: 10691528
PT journal article
AB Proteins capable of self-perpetuating changes in conformation and function (known as prions) can serve as genetic elements. To test whether novel prions could be created by recombinant methods, a yeast prion determinant was fused to the rat glucocorticoid receptor. The fusion protein existed in different heritable functional states, switched between states at a low spontaneous rate, and could be induced to switch by experimental manipulations. The complete change in phenotype achieved by transferring a prion determinant from one protein to another confirms the protein-only nature of prion inheritance and establishes a mechanism for engineering heritable changes in phenotype that should be broadly applicable.
MH Animal; Fungal Proteins/*chemistry/genetics/*metabolism; Genes, Reporter; Guanidine/pharmacology; Heat-Shock Proteins/pharmacology; Phenotype; Prions/*chemistry/genetics/*metabolism; Rats; Receptors, Glucocorticoid/chemistry/genetics/metabolism; Recombinant Fusion Proteins/chemistry/metabolism; Saccharomyces cerevisiae/chemistry/genetics; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.; Transcription, Genetic; Transformation, Genetic
AD Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, University of Chicago, 5841 South Maryland Avenue MC1028, Chicago, IL 60637, USA
SP englisch
PO USA