NR AHIW
AU Liberski,P.P.; Plucienniczak,A.; Hrabec,E.; Bogucki,A.
TI Ultrastructural studies of scrapie-associated fibrils and prion protein from hamster brains infected with scrapie
QU Folia Histochemica et Cytobiologica 1989; 27(1): 3-9
PT journal article
AB We report here about the purification of prion protein 27-30 (PrP 27-30) and scrapie-associated fibrils (SAF) from hamsters infected with the 263K strain of scrapie. Ultrastructural analysis of fractions from scrapie-infected brains revealed numerous fibrils measuring approximately 20 nm in diameter and 100-200 nm in length. The substructure of these fibrils consisted of protofilaments which were usually straight and rarely helically arranged. We conclude that the electron microscopic appearance of SAF depends much on the purification scheme.
MH Animal; Brain/pathology/*ultrastructure; Brain Chemistry; Electrophoresis, Polyacrylamide Gel; Female; Hamsters; Male; Mesocricetus; Microscopy, Electron; Molecular Weight; Myofibrils/microbiology/*ultrastructure; Prions/analysis/isolation & purification/*ultrastructure; Scrapie/*pathology; Sheep; Support, Non-U.S. Gov't
AD Department of Neurology, Academy of Medicine, Lodz, Poland.
SP englisch
PO Polen