NR AHMB
AU Liu,T.; Li,R.; Wong,B.S.; Liu,D.; Pan,T.; Petersen,R.B.; Gambetti,P.; Sy,M.S.
TI Normal cellular prion protein is preferentially expressed on subpopulations of murine hemopoietic cells
QU Journal of Immunology 2001 Mar 15; 166(6): 3733-42
ER J Immunol 2001 May 1;166(9):5840
PT journal article
AB We studied the expression of normal cellular prion protein (PrPc) in mouse lymphoid tissues with newly developed mAbs to PrPc. Most of the mature T and B cells in the peripheral lymphoid organs do not express PrPc. In contrast, most thymocytes are PrP(C+). In the bone marrow, erythroid cells and maturing granulocytes are PrP(C+). Approximately 50% of the cells in the region of small lymphocytes and progenitor cells also express PrPc. Most of these PrP(C+) cells are CD43(+), but B220(-), surface IgM(-) (sIgM(-)), and IL-7R(-), a phenotype that belongs to cells not yet committed to the B cell lineage. Another small group of the PrP(C+) cell are B220(+), and some of these are also sIgM(+). The majority of the B220(+) cells, however, are PrP(C-). Therefore, PrPc is preferentially expressed in early bone marrow progenitor cells and subsets of maturing B cells. Supporting this interpretation is our observation that stimulation of bone marrow cells in vitro with PMA results in a decrease in the number of PrP(C+)B220(-) cells with a corresponding increase of sIgM(+)B220(high) mature B cells. This result suggests that the PrP(C+)B220(-) cells are potential progenitors. Furthermore, in the bone marrow of Rag-1(-/-) mice, there are an increased number of PrP(C+)B220(-) cells, and most of the developmentally arrested pro-B cells in these mice are PrP(C+). Collectively, these results suggest that PrPc is expressed preferentially in immature T cells in the thymus and early progenitor cells in the bone marrow, and the expression of PrPc is regulated during hemopoietic differentiation.
MH Animal; Antigens, CD45/biosynthesis; B-Lymphocyte Subsets/cytology/metabolism; Bone Marrow Cells/cytology/drug effects/immunology/metabolism; Cell Differentiation/immunology; Cell Division/drug effects/immunology; Genes, RAG-1/immunology; Granulocytes/cytology/immunology/metabolism; Hematopoietic Stem Cells/cytology/*metabolism; Immunoglobulin M/biosynthesis; Immunoglobulins, Surface/biosynthesis; Lymphocyte Activation/drug effects; Lymphoid Tissue/cytology/*metabolism; Mice; Mice, Inbred C57BL; Mice, Knockout; PrPc Proteins/*biosynthesis/deficiency/genetics; Receptors, Interleukin-7/biosynthesis; Sialoglycoproteins/biosynthesis; Spleen/cytology/immunology/metabolism; T-Lymphocyte Subsets/cytology/metabolism; Tetradecanoylphorbol Acetate/pharmacology; Thymus Gland/cytology/immunology/metabolism
AD Institute of Pathology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
SP englisch
PO USA