NR AHQW
AU MacGregor,I.R.; Hope,J.; Barnard,G.; Kirby,L.; Drummond,O.; Pepper,D.; Hornsey,V.; Barclay,G.R.; Bessos,H.; Turner,M.L.; Prowse,C.V.
TI Application of a time-resolved fluoroimmunoassay for the analysis of normal prion protein in human blood and its components
QU Vox Sanguinis 1999; 77(2): 88-96
PT journal article
AB BACKGROUND AND OBJECTIVES: To quantify the cellular isoform of prion protein (PrPc) in human blood using a new time-resolved dissociation-enhanced fluoroimmunoassay (DELFIA). MATERIALS AND METHODS: The DELFIA was optimised for human blood samples and applied to isolated cell and plasma fractions from blood donations. The physicochemical properties of PrPc were analysed. RESULTS: 26. 5% of blood PrPc was associated with the platelet fraction, 0.8% with polymorphonuclear leucocytes, 2.4% with mononuclear leucocytes, 1.8% with red cells and 68.5% with plasma (mean values from 4 processed donations). CONCLUSION: The majority of blood PrPc is found in the platelet and plasma compartments.
MH Antigens; Blood Platelets/chemistry; Drug Stability; Erythrocytes/chemistry; Fluoroimmunoassay/methods; Human; Leukocytes, Mononuclear/chemistry; Neutrophils/chemistry; Plasma/chemistry; Prions/*blood/chemistry/immunology; Support, Non-U.S. Gov't
AD Ian MacGregor (ian.macgregor@snbts.csa.scot.nhs.uk), Olive Drummond, Duncan Pepper, Valerie Hornsey, Christopher Prowse (chris.prowse@snbts.csa.scot.nhs.uk), National Science Laboratory, Scottish National Blood Transfusion Service, Edinburgh; Robin Barclay, Hagop Bessos, Marc Turner, Academic Transfusion Medicine Unit, Scottish National Blood Transfusion Service, Edinburgh; Marc Turner, University of Edinburgh, Western General Hospital, Edinburgh; James Hope, Geoff Barnard, Louise Kirby, BBSRC Institute for Animal Health, Compton Laboratory, Newbury, UK
SP englisch
PO Schweiz