NR AHUG
AU Manuelidis,E.E.; Fritch,W.W.; Kim,J.H.; Manuelidis,L.
TI Immortality of cell cultures derived from brains of mice and hamsters infected with Creutzfeldt-Jakob disease agent
QU Proceedings of the National Academy of Sciences of the United States of America 1987 Feb; 84(3): 871-5
PT journal article
AB Isolates from six patients with Creutzfeldt-Jakob disease (CJD) were injected into various strains of hamsters and mice, and the infective agent was propagated. Serially passaged cultures were established from these CJD agent-infected brains and from uninfected control brains. All healthy cultures (21 out of 21) from CJD agent-infected brains became immortal and/or transformed. In contrast only 3 out of 13 normal brain cultures became immortal, and the rest died out with serial propagation in vitro. The fact that permanent cell lines were readily derived from multiple rodent strains and all CJD isolates tested suggests that a transforming capability is an intrinsic property of CJD agents. This conclusion is supported by demonstrations of in vitro cell transformation by CJD infectious brain fractions. Although the molecular mechanism of transformation events associated with the CJD agent is not presently known, a provocative possibility is that the CJD agent has a direct effect on the host genome by mechanisms analogous to those known for slowly oncogenic retroviruses.
IN 21 aus CJK-infizierten Hamster- oder Maushirnen gewonnene Zellkulturen wurden alle immortal, während dies bei nur 3 von 13 Zellkulturen aus nicht infizierten Gehirnen der Fall war.
MH Animal; Brain/*microbiology/pathology; Cell Survival; Cells, Cultured; Creutzfeldt-Jakob Syndrome/*microbiology/pathology; Hamsters; Human; Mice; Mice, Inbred Strains; Species Specificity; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.; Viruses/isolation & purification/ultrastructure
SP englisch
PO USA