NR AHWG
AU Markovits,P.; Dormont,D.; Delpech,B.; Court,L.; Latarjet,R.
TI [Trials of in vitro propagation of the scrapie agent in mouse nerve cells]
OT Essais de propagation in vitro de l'agent scrapie dans des cellules nerveuses de souris
QU Comptes Rendus des Seances de l'Academie des Sciences. Serie III, Sciences de la Vie 1981 Nov 2; 293(8): 413-7
PT journal article
AB We attempted to propagate the Scrapie agent in vitro in glia and neuroblastoma cells of Mice. Four out of seven assays of infection were positive, i.e. after several passages in vitro yielding at most a 10(9) fold final dilution of the original material, the extracts of each of the four cultures, when injected intracerebrally into CD1 Mice, produced a deadly disease displaying the clinical and pathological signs characteristic of Scrapie.
MH Animal; English Abstract; Mice; Mice, Inbred A; Neoplasms, Experimental; Neuroblastoma; Neuroglia; Prions/*physiology; Virus Cultivation; *Virus Replication
SP französisch
PO Frankreich