NR AHXT
AU Martin,T.; Hughes,S.; Hughes,K.; Dawson,M.
TI Direct sequencing of PCR amplified pig PrP genes
QU Biochimica et Biophysica Acta 1995 Apr 24; 1270(2-3): 211-4
PT journal article
AB The protein coding regions of the PrP genes of six pigs were sequenced directly from PCR-amplified genomic DNA. All six sequences were identical. The gene encodes a protein of 257 amino acids and shows an overall similarity of 77 to 88% with the PrP sequences from other mammalian species. The significance of amino acids unique to the pig PrP are discussed.
MH Amino Acid Sequence; Animal; Base Sequence; Cattle; Comparative Study; DNA Primers/genetics; Encephalopathy, Bovine Spongiform/genetics; Human; Molecular Sequence Data; Polymerase Chain Reaction; Prion Diseases/genetics/veterinary; Prions/*genetics; Protein Sorting Signals/genetics; Sequence Homology, Amino Acid; Sheep; Species Specificity; Swine; Swine Diseases/genetics
AD Central Veterinary Laboratory (Weybridge), New Hav, Addlestone, Surrey, UK
SP englisch
PO Niederlande