NR AHYX
AU Masison,D.C.; Maddelein,M.L.; Wickner,R.B.
TI The prion model for [URE3] of yeast: spontaneous generation and requirements for propagation.
QU Proceedings of the National Academy of Sciences of the United States of America 1997 Nov 11; 94(23): 12503-8
PT journal article
AB The genetic properties of the non-Mendelian element, [URE3], suggest that it is a prion (infectious protein) form of Ure2p, a mediator of nitrogen regulation in Saccharomyces cerevisiae. Into a ure2Delta strain (necessarily lacking [URE3]), we introduced a plasmid overproducing Ure2p. This induced the frequent "spontaneous generation" of [URE3], with properties identical to the original [URE3]. Altering the translational frame only in the prion-inducing domain of URE2 shows that it is Ure2 protein (and not URE2 RNA) that induces appearance of [URE3]. The proteinase K-resistance of Ure2p is unique to [URE3] strains and is not seen in nitrogen regulation of normal strains. The prion-inducing domain of Ure2p (residues 1-65) can propagate [URE3] in the absence of the C-terminal part of the molecule. In contrast, the C-terminal part of Ure2p cannot be converted to the prion (inactive) form without the prion-inducing domain covalently attached. These experiments support the prion model for [URE3] and extend our understanding of its propagation.
MH Amino Acid Sequence; Fungal Proteins/*genetics; *Gene Expression Regulation, Fungal; Molecular Sequence Data; Prions/*genetics; Saccharomyces cerevisiae/*genetics/growth & development
AD Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, Building 8, Room 225, National Institutes of Health, 8 Center Drive MSC0830, Bethesda, MD 20892-0830, USA
SP englisch
PO USA