NR AHZW
AU Masters,C.L.; Multhaup,G.; Simms,G.; Pottgiesser,J.; Martins,R.N.; Beyreuther,K.
TI Neuronal origin of a cerebral amyloid: neurofibrillary tangles of Alzheimer's disease contain the same protein as the amyloid of plaque cores and blood vessels.
QU EMBO Journal 1985 Nov; 4(11): 2757-63
PT journal article
AB The protein component of Alzheimer's disease amyloid [neurofibrillary tangles (NFT), amyloid plaque core and congophilic angiopathy] is an aggregated polypeptide with a subunit mass of 4 kd (the A4 monomer). Based on the degree of N-terminal heterogeneity, the amyloid is first deposited in the neuron, and later in the extracellular space. Using antisera raised against synthetic peptides, we show that the N terminus of A4 (residues 1-11) contains an epitope for neurofibrillary tangles, and the inner region of the molecule (residues 11-23) contains an epitope for plaque cores and vascular amyloid. The non-protein component of the amyloid (aluminum silicate) may form the basis for the deposition or amplification (possible self-replication) of the aggregated amyloid protein. The amyloid of Alzheimer's disease is similar in subunit size, composition but not sequence to the scrapie-associated fibril and its constituent polypeptides. The sequence and composition of NFT are not homologous to those of any of the known components of normal neurofilaments.
MH Alzheimer Disease/*metabolism/pathology; Amino Acids/analysis; Amyloid/*analysis; Brain/pathology; *Brain Chemistry; Human; Immunoassay; Lipofuscin/isolation & purification; Macromolecular Systems; Molecular Weight; Neurofibrils/*analysis; Support, Non-U.S. Gov't
SP englisch
PO England