NR AIHO
AU Merz,P.A.; Wisniewski,H.M.; Somerville,R.A.; Bobin,S.A.; Masters,C.L.; Iqbal,K.
TI Ultrastructural morphology of amyloid fibrils from neuritic and amyloid plaques
QU Acta Neuropathologica 1983; 60(1-2): 113-24
PT journal article
AB The structure of partially purified, CNS amyloid fibrils from three different sources have been compared by negative stain EM. The fibrils isolated from brains with senile dementia of Alzheimer type were 4-8 nm in diameter, narrowing every 30-40 nm and apparently composed of two 2-4 nm filaments. The fibrils from a Gerstmann-Sträussler syndrome brain were 7-9 nm in diameter, narrowing every 70-80 nm and with a suggestion that they are composed of two 3-5 nm filaments. The fibrils isolated from 87V scrapie-affected mouse brains were 4-8 nm in diameter with a twist every 15-25 nm presumably composed of two 2-4 nm filaments. The fibrils from the scrapie brains were usually observed in pairs. The shape of the clusters of the isolated amyloid fibrils observed in each disease was similar in negative stain and thin section EM preparations and was related to the characteristic morphology of the amyloid fibrils in the neuritic and amyloid plaques in situ. The structural differences between the CNS amyloid fibrils from the various diseases studied by us may reflect differences in the polypeptides which comprise the fibril and/or a different pathogenesis in the formation of the amyloid fibrils.
IN Während die verdrillten amyloiden Strukturen in Alzheimer-Gehirnen bei 4-8 mm Durchmesser auf einer Strecke von 30-40 nm eine halbe Drehung ausführen und anscheinend aus 2-4 Filamenten bestehen, hatten die Fibrillen aus dem Gehirn eines Gerstmann-Sträussler-Patienten Durchmesser von 7-9 nm, maßen zwischen den scheinbaren Einschnürungen nach jeder halben Umdrehung 70-80 nm und schienen aus 3-5 Filamenten zu bestehen. Die Fibrillen aus mit 87V-Scrapie infizierten Maushirnen hatten Durchmesser von 4-8 nm, erreichten eine halbe Drehung nach 15-25 nm und schienen aus 2-4 Filamenten zu bestehen. Auch die Form der Amyloid-Cluster war jeweils charakteristisch für die jeweilige Krankheit.
MH Alzheimer Disease/*pathology; *Amyloid; Animal; Brain/ultrastructure; Brain Diseases/*pathology; Dementia/*pathology; Human; Mice; Microscopy, Electron; Scrapie/*pathology; Sheep
SP englisch
PO Westdeutschland