NR AINL
AU Montrasio,F.; Cozzio,A.; Flechsig,E.; Rossi,D.; Klein,M.A.; Rülicke,T.; Raeber,A.J.; Vosshenrich,C.A.; Proft,J.; Aguzzi,A.; Weissmann,C.
TI B lymphocyte-restricted expression of prion protein does not enable prion replication in prion protein knockout mice
QU Proceedings of the National Academy of Sciences of the United States of America 2001 Mar 27; 98(7): 4034-7
PT journal article
AB Prion replication in spleen and neuroinvasion after i.p. inoculation of mice is impaired in forms of immunodeficiency where mature B lymphocytes are lacking. In spleens of wild-type mice, infectivity is associated with B and T lymphocytes and stroma but not with circulating lymphocytes. We generated transgenic prion protein knockout mice overexpressing prion protein in B lymphocytes and found that they failed to accumulate prions in spleen after i.p. inoculation. We conclude that splenic B lymphocytes are not prion-replication competent and that they acquire prions from other cells, most likely follicular dendritic cells with which they closely associate and whose maturation depends on them.
MH Animal; B-Lymphocytes/*metabolism; Central Nervous System/metabolism; Dendritic Cells, Follicular/metabolism; Mice; Mice, Knockout; Mice, Transgenic; Prions/genetics/*metabolism; Spleen/metabolism; Support, Non-U.S. Gov't
AD Institute of Molecular Biology, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.
SP englisch
PO USA