NR AIOB
AU Moreau-Dubois,M.C.; Brown,P.; Rohwer,R.G.; Masters,C.L.; Franko,M.; Gajdusek,D.C.
TI Experimental scrapie in golden Syrian hamsters: temporal comparison of in vitro cell-fusing activity with brain infectivity and histopathological changes.
QU Infection and Immunity 1982 Jul; 37(1): 195-9
PT journal article
AB Golden Syrain hamsters were inoculated intracerebrally with the hamster-adapted 263K strain of scrapie virus, and the evolution of in vitro cell fusing activity induced by brain suspensions was compared with brain infectivity titers and histological changes. Cell-fusing activity abruptly appeared 4 weeks after inoculation, 1 week before the earliest detectable histopathological changes, at an infectivity level of 7.6 log 50% lethal doses per g of brain. Cell-fusing activity was sustained throughout the remaining 4 weeks of the incubation period and the subsequent 1- to 3-week stage of clinical illness but did not increase with the logarithmic progression of infectivity, which reached a level of 11 log 50% lethal doses per g in the agonal stage of disease. Gliosis was most sensitively detected by a monoclonal antibody reacting with astrocyte intermediate filaments in an indirect immunofluorescence test, anticipating histological recognition of gliosis and spongiform change by 1 to 2 weeks. In vitro cell-fusing activity is thus one of the earliest known biological markers (apart from infectivity itself) of experimental scrapie infection.
IN Scrapie-infizierte Hamstergehirne weisen eine Zellfusionsaktivität auf, die später als die Infektiosität des Gewebes aber früher als die ersten histopathologischen Veränderungen feststellbar ist. Die Zunahme dieser Zellfusionsaktivität korreliert allerdings nicht mit der Zunahme der Infektiosität.
MH Animal; Astrocytes/pathology; Brain/microbiology/*pathology; Cell Fusion; Female; Gliosis/pathology; Hamsters; Mesocricetus; Prions/*physiology; Scrapie/*microbiology/pathology; Sheep; Time Factors
SP englisch
PO USA