NR AIPR
AU Mouillet-Richard,S.; Ermonval,M.; Chebassier,C.; Laplanche,J.L.; Lehmann,S.; Launay,J.M.; Kellermann,O.
TI Signal transduction through prion protein
QU Science 2000 Sep 15; 289(5486): 1925-8
PT journal article
AB The cellular prion protein PrPc is a glycosylphosphatidylinositol-anchored cell-surface protein whose biological function is unclear. We used the murine 1C11 neuronal differentiation model to search for PrPc-dependent signal transduction through antibody-mediated cross-linking. A caveolin-1-dependent coupling of PrPc to the tyrosine kinase Fyn was observed. Clathrin might also contribute to this coupling. The ability of the 1C11 cell line to trigger PrPc-dependent Fyn activation was restricted to its fully differentiated serotonergic or noradrenergic progenies. Moreover, the signaling activity of PrPc occurred mainly at neurites. Thus, PrPc may be a signal transduction protein.
MH Animal; Cell Compartmentation; Cell Differentiation; Enzyme Activation; Membrane Proteins/metabolism; Mice; Neurons/cytology/metabolism; PrPc Proteins/*metabolism; Proto-Oncogene Proteins/metabolism; *Signal Transduction; Support, Non-U.S. Gov't
AD Differenciation Cellulaire, CNRS-Institut Pasteur, 75724 Paris Cedex 15, France. srichard@pasteur.fr
SP englisch
PO USA