NR AIQN
AU Multhaup,G.; Diringer,H.; Hilmert,H.; Prinz,H.; Heukeshoven,J.; Beyreuther,K.
TI The protein component of scrapie-associated fibrils is a glycosylated low molecular weight protein
QU EMBO Journal 1985 Jun; 4(6): 1495-501
PT journal article
AB Scrapie-associated fibril protein (SAF-protein) extracted from infectious scrapie-associated fibrils (SAF) isolated from scrapie hamster brains is not infectious. SAF-protein is composed of various mol. wt. species of glycoproteins differing in carbohydrate content rather than amino acid composition. The N-linked carbohydrate chains represent approximately 40-60% of the mol. wt. of SAF-protein. The deglycosylated SAF-protein has a surprisingly low mol. wt. of approximately 7 kd, representing approximately 55 amino acid residues. This size and chemical analyses indicate that SAF-protein is an amyloid-type of protein. The simplest explanation for the available data is that SAF-polypeptide is very likely not to be part of the scrapie agent but that it is, like other amyloid proteins, derived from host-encoded proteins and not infectious. It is suggested that the infectivity of fractions rich in SAF is due to co-purification of scrapie virus and SAF caused by the high carbohydrate content of SAF-protein.
MH Amino Acid Sequence; Amino Acids/analysis; Animal; Glycoproteins/*analysis; Hamsters; Molecular Weight; Nerve Tissue Proteins/*analysis/isolation & purification; PrP 27-30 Protein; Support, Non-U.S. Gov't
SP englisch
PO England