NR AIST
AU Nakayashiki,T.; Ebihara,K.; Bannai,H.; Nakamura,Y.
TI Yeast [PSI+] "prions" that are crosstransmissible and susceptible beyond a species barrier through a quasi-prion state
QU Molecular Cell 2001 Jun; 7(6): 1121-30
PT journal article
AB The yeast [PSI(+)] element represents an aggregated form of release factor Sup35p and is inherited by a prion mechanism. A "species barrier" prevents crosstransmission of the [PSI(+)] state between heterotypic Sup35p "prions." Kluyveromyces lactis and Yarrowia lipolytica Sup35 proteins, however, show interspecies [PSI(+)] transmissibility and susceptibility and a high spontaneous propagation rate. Cross-seeding was visualized by coaggregation of differential fluorescence probes fused to heterotypic Sup35 proteins. This coaggregation state, referred to as a "quasi-prion" state, can be stably maintained as a heritable [PSI(+)] element composed of heterologous Sup35 proteins. K. lactis Sup35p was capable of forming [PSI(+)] elements not only in S. cerevisiae but in K. lactis. These two Sup35 proteins contain unique multiple imperfect oligopeptide repeats responsible for crosstransmission and high spontaneous propagation of novel [PSI(+)] elements.
MH Candida/metabolism; Fungal Proteins/*genetics/*metabolism; Genes, Fungal/*physiology; Genes, Reporter; Indicators and Reagents/metabolism; Kluyveromyces; Luminescent Proteins/genetics; Membrane Fusion; Molecular Sequence Data; Prions/metabolism; Repetitive Sequences, Nucleic Acid; Saccharomyces cerevisiae/metabolism; Species Specificity; Support, Non-U.S. Gov't; Transformation, Genetic; Yeasts/*metabolism; Zygosaccharomyces/metabolism
AD Department of Basic Medical Sciences and, Institute of Medical Science, University of Tokyo, 108-8639, Tokyo, Japan. nak@ims.u-tokyo.ac.jp
SP englisch
PO USA