NR AITV
AU Narang,H.K.
TI Detection of single-stranded DNA in scrapie-infected brain by electron microscopy
QU Journal of Molecular Biology 1990 Dec 5; 216(3): 469-73
PT journal article
AB The nucleic acid content of enriched preparations of mitochondria/tubulofilamentous particles from normal and scrapie-infected hamster brains were examined by electron microscopy. After spreading on collodion-coated grids circular molecules of approximately 15.7 kb corresponding in size to mitochondrial DNA (mtDNA) were observed both in normal and scrapie-infected brains. In nucleic acid preparations from scrapie-infected brains multimeric mtDNA and single-stranded DNA strands of about 0.49 X 10(6) daltons were also visualized. These findings demonstrate the presence of a single-stranded DNA in scrapie-infected brains and are consistent with previous data based on enzyme digestion of nucleic acids isolated from scrapie-infected brains.
IN Nur in scrapieinfizierten Hamstergehirnen glaubt der Autor eine einzelsträngige DNA mit einer Molekularmasse von 490 Kilodalton gefunden zu haben.
MH Animal; Brain/pathology; *Brain Chemistry; DNA, Single-Stranded/*analysis/ultrastructure; Female; Hamsters; Mesocricetus; Microscopy, Electron/methods; Scrapie/*genetics; Support, Non-U.S. Gov't
AD Institute of Pathology, General Hospital, Newcastle Upon Tyne, England.
SP englisch
PO England
OR Prion-Krankheiten 6