NR AIVE
AU Neibergs,H.L.; Ryan,A.M.; Womack,J.E.; Spooner,R.L.; Williams,J.L.
TI Polymorphism analysis of the prion gene in BSE-affected and unaffected cattle
QU Animal Genetics 1994 Oct; 25(5): 313-7
PT journal article
AB Polymerase chain reaction (PCR) primers designed to amplify the octapeptide repeat region of the bovine prion gene were used to test the association of genotypes with bovine spongiform encephalitis (BSE) in 56 BSE-affected and 177 unaffected animals. Three alleles (A,B,C) were detected as single-strand conformation polymorphisms (SSCPs) and two alleles (1,2 - representing six or five copies of the octapeptide repeat respectively) were detected as amplified double-strand fragment length polymorphisms (AMFLPs). Observed genotypes of SSCPs and AMFLPs were analysed by chi-square. The SSCP genotypes of nuclear family members of animals with BSE and BSE-affected animals were different (P < 0.001, P < 0.01) from unrelated animals of the same breed without BSE. No genotypic differences were found between the BSE-affected animals and their relatives (P > 0.469). No AMFLP genotypic differences were detected between BSE-affected animals, their relatives, unrelated animals of the same breed or animals of different breeds (P > 0.05). These data suggest that BSE-affected animals and their relatives are more likely to have the AA SSCP genotype than unrelated animals of the same breed or animals of different breeds.
IN Die Sequenzen der Oktapeptid-Regionen der Prionproteine BSE-kranker englischer Rinder und ihrer engsten Angehörigen sollen sich nach Ansicht der Autoren statistisch signifikant häufig von denen nicht erkrankter Tiere der selben Herden unterscheiden. Auch bei Rindern scheint es also in Abhängigkeit von der Form des Prionproteins unterschiedliche Empfänglichkeiten für die Erkrankung an BSE zu geben.
ZR 17
MH Alleles; Animal; Base Sequence; Cattle/*genetics; Comparative Study; DNA/blood; DNA Primers; Encephalopathy, Bovine Spongiform/*genetics; Genotype; Lymphocytes/cytology; Molecular Sequence Data; Polymerase Chain Reaction; *Polymorphism (Genetics); Prions/*genetics; Reference Values; Species Specificity; Support, Non-U.S. Gov't; Support, U.S. Gov't, Non-P.H.S.
AD Department of Veterinary Pathobiology, Texas A&M University, College Station 77843.
SP englisch
PO England
OR Prion-Krankheiten 6