NR AIZT
AU Oesch,B.; Westaway,D.; Walchli,M.; McKinley,M.P.; Kent,S.B.; Aebersold,R.; Barry,R.A.; Tempst,P.; Teplow,D.B.; Hood,L.E.; Prusiner,S.B.; Weissmann,C.
TI A cellular gene encodes scrapie PrP 27-30 protein
QU Cell 1985 Apr; 40(4): 735-46
PT journal article
AB A clone encoding PrP 27-30, the major protein in purified preparations of scrapie agent, was selected from a scrapie-infected hamster brain cDNA library by oligonucleotide probes corresponding to the N terminus of the protein. Southern blotting with PrP cDNA revealed a single gene with the same restriction patterns in normal and scrapie-infected brain DNA. A single PrP-related gene was also detected in murine and human DNA. PrP-related mRNA was found at similar levels in normal and scrapie-infected hamster brain, as well as in many other normal tissues. Using antisera against PrP 27-30, a PrP-related protein was detected in crude extracts of infected brain and to a lesser extent in extracts of normal brain. Proteinase K digestion yielded PrP 27-30 in infected brain extract, but completely degraded the PrP-related protein in normal brain extract. No PrP-related nucleic acids were found in purified preparations of scrapie prions, indicating that PrP 27-30 is not encoded by a nucleic acid carried within the infectious particles.
IN Mit Oligonukleotiden wurde ein Prionprotein-Klon aus einer Hamster-cDNA-Bibliothek gefischt. Damit konnte auf Southern blots ein einzelnes Gen markiert werden. Die mRNA des Prionproteins war in normalem Gewebe ebenso häufig wie in infiziertem Gewebe.
MH Amino Acid Sequence; Animal; Base Sequence; Brain Chemistry; Cloning, Molecular; DNA/*analysis; Hamsters; Human; Mice; Nucleic Acid Hybridization; Nucleic Acids/analysis; Poly A/genetics/isolation & purification; PrP 27-30 Protein; Prions; RNA, Messenger/genetics/isolation & purification; Scrapie/*genetics; Sheep; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.; Viral Proteins/*analysis/*genetics
AD Prof. Charles Weissmann, Universität Zürich, Institut für Molekularbiologie, Abteilung I, Hönggerberg, 8093 Zürich, Schweiz, Postfach, Tel. 633 24 91, Fax 371 72 05
SP englisch
PO USA
OR Prion-Krankheiten 6