NR AJIN
AU Peoc'h,K.; Schröder,H.C.; Laplanche,J.L.; Ramljak,S.; Müller,W.E.G.
TI Determination of 14-3-3 protein levels in cerebrospinal fluid from Creutzfeldt-Jakob patients by a highly sensitive capture assay
QU Neuroscience Letters 2001 Apr 6; 301(3): 167-70
PT journal article
AB The level of 14-3-3gamma protein was determined in the cerebrospinal fluid (CSF) from patients with Creutzfeldt-Jakob disease (CJD) and non-CJD patients applying a new and fast microplate assay (14-3-3 protein capture assay), based on the binding to a peptide comprising a phosphorylated recognition motif of 14-3-3 protein. The levels of the gamma-isoform of 14-3-3 protein in CSF samples from CJD patients (n=41) were significantly higher than those observed in patients with non-CJD dementias (n=36) suggesting that this capture assay is a reliable method in the diagnosis of CJD. Since this assay allows the direct measurement of 14-3-3 protein in the CSF without prior concentration it is an easy and simple alternative to the conventionally applied immunoblotting procedures.
MH Chemistry, Clinical/*methods; Creutzfeldt-Jakob Syndrome/*cerebrospinal fluid/*diagnosis; Enzyme-Linked Immunosorbent Assay/*methods; Human; Immunoblotting; Phosphorylation; Protein Binding; Sensitivity and Specificity; Support, Non-U.S. Gov't; Tyrosine 3-Monooxygenase/*cerebrospinal fluid
AD CR C. Bernard, Service de Biochimie et Biologie Moleculaire, Hopital Lariboisiere, 2, rue A. Pare, F-75010, Paris, France.
SP englisch
PO Irland