NR AJMZ
AU Poleshchuk,N.N.; Il'kevich,Y.G.; Kapitulets,S.P.; Kvacheva,Z.B.; Luchko,V.P.; Kolomiets,N.D.; Votiakov,V.I.
TI [The formation of infectious scrapie-like structures in the persistence of the agent of amyotrophic leukospongiosis in a brain cell culture]
OT Formirovanie infektsionnykh skrepipodobnykh struktur pri persistentsii agenta amiotroficheskogo leikospongioza v kul'ture kletok mozga
QU Voprosy Virusologii 1991 Jan-Feb; 36(1): 37-40
PT journal article
AB Electron microscopic analysis of specimens from guinea-pig brain cell cultures infected with amyotrophic leucospongiosis agent (belonging to "unconventional" viruses) revealed accumulation in the culture fluid of abnormal filamentous structures similar to scrapie-associated fibrils (SAF) differing in morphology. Most of these SAF-like structures 10-15 nm in diameter contained helically wound protofilaments with a repeat at certain intervals (50-150 nm). When these structures were inoculated into guinea-pig brain astrocyte cultures they produced dystrophic-destructive changes in some (25%) astrocytes, and their intracerebral inoculation to guinea pigs produced an experimental disease. The abnormal SAF-like structures were reisolated from the brains of the inoculated animals which indicated the relationship between these structures and infectivity.
MH Animal; Astrocytes/microbiology; Brain/cytology/*microbiology; Brain Diseases/*microbiology; Comparative Study; English Abstract; Guinea Pigs; Microscopy, Electron; *Motor Neurons; Neuromuscular Diseases/*microbiology; Prions/*isolation & purification/pathogenicity/ultrastructure; Slow Virus Diseases/*microbiology; Virus Cultivation
SP russisch
PO Sowjetunion