NR AJUH
AU Race,R.E.; Jenny,A.; Sutton,D.
TI Scrapie infectivity and proteinase K-resistant prion protein in sheep placenta, brain, spleen, and lymph node: implications for transmission and antemortem diagnosis.
QU Journal of Infectious Diseases 1998 Oct; 178(4): 949-53
IA http://www.journals.uchicago.edu/JID/journal/issues/v178n4/oc25_949/oc25_949.web.pdf?erFrom=-6776443405321166708Guest
PT journal article
AB Probable transmission of bovine spongiform encephalopathy to humans has focused intense interest on all of the transmissible spongiform encephalopathies (TSEs) and how they spread. In all TSEs, an abnormal disease-associated, proteinase K-resistant protein referred to as PrPres or PrPsc accumulates in brain. In some species, PrPres accumulates in other tissues as well. Sheep placenta, brain, spleen, and lymph node were analyzed in detail for PrPres and infectivity. Both were detected in all brain and spleen samples and in placenta and lymph nodes of 80% of the scrapie-infected sheep. A perfect correlation was observed between infectivity and PrPres detection. These results substantiate the probability that placenta plays an important role in natural transmission of scrapie, suggest that analysis of placenta for PrPres could be the basis for an antemortem test for sheep scrapie, and show that PrPres, scrapie infectivity, and scrapie disease are closely associated.
IN Bei Scrapie-infizierten Schafen fanden die Autoren PrPres und per Bioassay auch Infektiosität immer in Hirn und Milz sowie bei 80% der Tiere außerdem in Lymphknoten und Plazenta.
MH Animal; Brain Chemistry; Endopeptidase K/metabolism; Female; Lymph Nodes/chemistry; Placenta/*chemistry; PrPsc Proteins/*isolation & purification/metabolism; Scrapie/*diagnosis/*transmission; Sheep; Spleen/chemistry; Tissue Distribution
AD Richard Race (Rrace@atlas.niaid.nih.gov), Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana, 59840, USA; Allen Jenny, US Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services, National Veterinary Services Laboratories, Ames, Iowa; Diane Sutton, US Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services, Riverdale, Maryland
SP englisch
PO USA