NR AJUJ
AU Race,R.E.; Ernst,D.; Jenny,A.; Taylor,W.; Sutton,D.; Caughey,B.W.
TI Diagnostic implications of detection of proteinase K-resistant protein in spleen, lymph nodes, and brain of sheep
QU American Journal of Veterinary Research 1992 Jun; 53(6): 883-9
PT journal article
AB Brain, spleen, and selected lymph nodes from sheep with clinical signs of scrapie were analyzed for presence of proteinase K-resistant protein (PrPres). Diagnosis of scrapie on the basis of detection of PrPres was compared with diagnosis on the basis of histologic evaluation of the brain from clinically affected or exposed sheep. Proteinase K-resistant protein was found in every brain that was histologically positive for scrapie, and in addition, was found in the brain of several clinically positive sheep that were not diagnosed as scrapie-positive by histologic evaluation. Proteinase K-resistant protein was also found in 87% of the spleens and lymph nodes from sheep that had PrPres detected in brain homogenates. Therefore, analysis of sheep brain, spleen, or lymph nodes for PrPres provided a diagnostic approach that was superior to histologic examination alone for detection of naturally scrapie agent-infected sheep.
IN Der Nachweis proteaseresistenter Prionproteine in Gehirn, Milz und Lymphknoten von Schafen stellt ein sichereres Nachweisverfahren für Scrapie dar. In vielen Fällen konnte die klinische Diagnose so, aber nicht histopathologisch bestätigt werden.
MH Animal; Brain/pathology; *Brain Chemistry; Comparative Study; Immunoblotting; Lymph Nodes/*chemistry; Membrane Glycoproteins/analysis; PrPsc Proteins; Prions/*analysis; Scrapie/*diagnosis; Sheep; Spleen/*chemistry
AD National Institutes of Health, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, Hamilton, MT 59480.
SP englisch
PO USA