NR AJUP
AU Race,R.E.; Ernst,D.
TI Detection of proteinase K-resistant prion protein and infectivity in mouse spleen by 2 weeks after scrapie agent inoculation
QU Journal of General Virology 1992 Dec; 73(12): 3319-23
PT journal article
AB The sequential accumulation of the protease-resistant form of the endogenous prion protein (PrPres) was compared to levels of scrapie infectivity in the spleen and brain of scrapie-infected mice at various times after inoculation. In mouse spleen PrPres was detected 1 week after inoculation, and increased 65-fold between 1 and 3 weeks post-inoculation and an additional 15-fold during the next 17 weeks. Infectivity in spleen reached a maximum plateau level by 3 weeks. In contrast, in mouse brain PrPres was not detected until 8 weeks after inoculation and then increased 200-fold during the next 12 weeks. During this same time, infectivity increased approximately 10,000-fold. Therefore, in both spleen and brain of scrapie-infected mice accumulation of PrPres and infectivity appear to be associated. However, it was not possible to show quantitative correlations between PrPres detection and infectivity, perhaps owing to the inaccuracy of the infectivity assay.
IN In mit Scrapie infizierten Mäusen ist die Protease K - resistente Form des Prionproteins in der Milz nach 1 Woche nachweisbar und nimmt zwischen der 1. und der 3. Woche 65-fach und während der nächsten 17 Wochen noch einmal 15-fach zu. Die Infektivität der Milz erreicht nach 3 Wochen ihr Maximum. In Mausgehirnen ist die Protease K - resistente Form des Prionproteins erst nach 8 Woche nachweisbar und nimmt während der nächsten 12 Wochen 200-fach zu. Die Infektivität des Gehirnes nimmt während dieser Zeit rund 10.000-fach zu. Die Akkumulation des Prionproteins und das Infektionspotential korrelieren demnach qualitativ, aber nicht quantitativ.
MH Animal; Brain Chemistry; Endopeptidase K; Mice; PrPsc Proteins; Prions/*chemistry/metabolism; Scrapie/physiopathology/*transmission; Serine Endopeptidases/pharmacology; Spleen/chemistry; Time Factors
AD National Institutes of Health, National Institute of Allergy and Infectious Diseases, Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, Hamilton, Montana 59840.
SP englisch
PO England