NR AKHJ
AU Saez-Valero,J.; Angeretti,N.; Forloni,G.
TI Caspase-3 activation by beta-amyloid and prion protein peptides is independent from their neurotoxic effect
QU Neuroscience Letters 2000 Nov 3; 293(3): 207-10
PT journal article
AB Synthetic peptides corresponding to residues 25-35 of beta-amyloid (beta 25-35) and 106-126 of prion protein (PrP 106-126) are amyloidogenic and cause neuronal death by apoptosis in vitro. We evaluated, in rat cortical neurons, the role of caspases activation in the peptides neurotoxicity by measuring of caspase-3 (CPP32) activity and applying a non-selective caspase inhibitor (z-VAD-fmk) or CPP32-specific inhibitor (Asp-Glu-Val-Asp-CHO (DEVD-CHO)). CPP32 was dose-dependently activated by both peptides (2.5-50 microM). The caspase inhibitors completely abolished the CPP32 activation induced by the peptides. However, the neurotoxic effect was partially attenuated with z-VAD-fmk, while no antagonism was found with DEVD-CHO. Thus, although beta 25-35 and PrP 106-126 robustly activated CPP32, their neurotoxic effect was independent of this caspase activation.
MH Amino Acid Chloromethyl Ketones/pharmacology; Amyloid beta-Protein/*pharmacology/toxicity; Animal; Apoptosis; Caspases/antagonists & inhibitors/*metabolism; Cell Survival/drug effects; Cells, Cultured; Cerebral Cortex/cytology/drug effects/enzymology; Cysteine Proteinase Inhibitors/pharmacology; Dose-Response Relationship, Drug; Enzyme Activation/drug effects; Neurons/cytology/*drug effects/*enzymology; Oligopeptides/pharmacology; Peptide Fragments/*pharmacology/toxicity; Prions/*pharmacology/toxicity; Rats; Support, Non-U.S. Gov't
AD Biology of Neurodegenerative Disorders, Istituto di Ricerche Farmacologiche 'Mario Negri Via Eritrea 62, 20157, Milan, Italy.
SP englisch
PO Irland