NR AKRQ
AU Semancik,J.S.; Marsh,R.F.; Geelen,J.L.; Hanson,R.P.
TI Properties of the scrapie agent-endomembrane complex from hamster brain
QU Journal of Virology 1976 May; 18(2): 693-700
PT journal article
AB Subcellular fractionation of scrapie-infected hamster brain indicated the association of the scrapie agent with a component of the endomembrane system. Characterization by equilibrium density gradient centrifugation, electron microscopy, and marker enzymes suggested a primary association with rough and smooth endoplasmic reticulum and a possible incorporation into the plasma membrane. DNA polymerase activity demonstrated a direct correlation with regions of scrapie activity from the gradient fractions. A scrapie-related product was detected after (3H)TMP incorporation and analysis on 2.2% polyacrylamide gels. Analysis of nucleic acid species extracted from subcellular fractions resulted in a greater quantity from healthy brain; however, no qualitative distinctions were detected.
MH Adenosinetriphosphatase/metabolism; Animal; Brain/enzymology/*microbiology; Cell Membrane/enzymology/microbiology; Cytochrome Reductases/metabolism; DNA Nucleotidyltransferases/metabolism; Endoplasmic Reticulum/enzymology/*microbiology; Hamsters; Nucleic Acids/analysis; *Prions/analysis/ultrastructure; Subcellular Fractions; Support, U.S. Gov't, Non-P.H.S.; Support, U.S. Gov't, P.H.S.; Viral Proteins/analysis
SP englisch
PO USA