NR AKUJ
AU Shinagawa,M.; Munekata,E.; Doi,S.; Takahashi,K.; Goto,H.; Sato,G.
TI Immunoreactivity of a synthetic pentadecapeptide corresponding to the N-terminal region of the scrapie prion protein
QU Journal of General Virology 1986 Aug; 67(8): 1745-50
PT journal article
AB A pentadecapeptide with an amino acid sequence corresponding to the amino-terminal region of the scrapie prion protein was synthesized. Immunization of a rabbit with the peptide conjugated with ovalbumin induced specific antibodies. The antibodies reacted with all three of the major polypeptides in a proteinase K-treated fraction obtained from brains of mice infected with the Obihiro strain of scrapie agent. Some peptides in the proteinase-untreated fraction also shared antigenicity with the three major polypeptides. Specific polypeptides were also detected by the antiserum in a fraction prepared from spleens, but only two of the three major polypeptides were found and the amounts of the polypeptides were less than in brain.
MH Animal; Antibodies, Viral/biosynthesis; Antigens, Viral/*immunology; Brain Chemistry; Mice; Peptides/analysis/chemical synthesis/*immunology; Prions/*immunology; Scrapie/metabolism/microbiology; Spleen/analysis; Support, Non-U.S. Gov't; Viral Proteins/analysis/*immunology
SP englisch
PO England