NR AKWB
AU Sigurdson,C.J.; Williams,E.S.; Miller,M.W.; Spraker,T.R.; O'Rourke,K.I.; Hoover,E.A.
TI Oral transmission and early lymphoid tropism of chronic wasting disease PrPres in mule deer fawns (Odocoileus hemionus)
QU Journal of General Virology 1999 Oct; 80(10): 2757-64
IA http://intl-vir.sgmjournals.org/cgi/content/full/80/10/2757
PT journal article
AB Mule deer fawns (Odocoileus hemionus) were inoculated orally with a brain homogenate prepared from mule deer with naturally occurring chronic wasting disease (CWD), a prion-induced transmissible spongiform encephalopathy. Fawns were necropsied and examined for PrPres, the abnormal prion protein isoform, at 10, 42, 53, 77, 78 and 80 days post-inoculation (p.i.) using an immunohistochemistry assay modified to enhance sensitivity. PrPres was detected in alimentary-tract-associated lymphoid tissues (one or more of the following: retropharyngeal lymph node, tonsil, Peyer's patch and ileocaecal lymph node) as early as 42 days p.i. and in all fawns examined thereafter (53 to 80 days p.i.). No PrPres staining was detected in lymphoid tissue of three control fawns receiving a control brain inoculum, nor was PrPres detectable in neural tissue of any fawn. PrPres-specific staining was markedly enhanced by sequential tissue treatment with formic acid, proteinase K and hydrated autoclaving prior to immunohistochemical staining with monoclonal antibody F89/160.1.5. These results indicate that CWD PrPres can be detected in lymphoid tissues draining the alimentary tract within a few weeks after oral exposure to infectious prions and may reflect the initial pathway of CWD infection in deer. The rapid infection of deer fawns following exposure by the most plausible natural route is consistent with the efficient horizontal transmission of CWD in nature and enables accelerated studies of transmission and pathogenesis in the native species.
MH Animal; Chronic Disease; Deer; Lymphoid Tissue/*metabolism; Prion Diseases/etiology/pathology/*veterinary; Prions/*analysis; Staining and Labeling; Support, Non-U.S. Gov't; Time Factors; Tissue Distribution; Wasting Syndrome/etiology/pathology/veterinary
AD
Christina J. Sigurdson,1 Elizabeth S. Williams,2 Michael W. Miller,3 Terry R. Spraker,1,4 Katherine I. O'Rourke5 and Edward A. Hoover1
1 Department of Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523-1671, USA
2 Department of Veterinary Sciences, University of Wyoming, 1174 Snowy Range Road, University of Wyoming, Laramie, WY 82070, USA
3 Colorado Division of Wildlife, Wildlife Research Center, 317 West Prospect Road, Fort Collins, CO 80526-2097, USA
4 Colorado State University Veterinary Diagnostic Laboratory, 300 West Drake Road, Fort Collins, CO 80523-1671, USA
5 Animal Disease Research Unit, Agricultural Research Service, US Department of Agriculture, 337 Bustad Hall, Washington State University, Pullman, WA 99164-7030, USA
Author for correspondence: Edward Hoover. Fax +1 970 491 0523. e-mail ehoover@lamar.colostate.edu
SP englisch
PO England
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