NR AKWR
AU Simmons,M.M.; Blamire,I.W.H.; Austin,A.R.
TI Simple method for the perfusion-fixation of adult bovine brain
QU Research in Veterinary Science 1996 May; 60(3): 247-50
PT journal article
AB It is widely accepted that the vascular perfusion of a tissue leads to its optimal fixation for ultrastructural examination, but the technique has been applied with little success in large animals such as cattle. This paper describes a simple and highly reproducible method for the perfusion-fixation of adult bovine brain. The carotid arteries are cannulated and the perfusate is introduced by means of a peristaltic pump. The inclusion of toluidine blue in the fixative makes it possible to assess the success of the technique, and prevents the costly and time-consuming processing of material which is suboptimally fixed. The method is potentially valuable for the examination of a wide range of neurological conditions in large domestic animals at an ultrastructural level in optimally fixed material.
MH Animal; Brain/cytology/*ultrastructure; Cattle; Cerebrovascular Circulation; Dyes; Encephalopathy, Bovine Spongiform/pathology; Histological Techniques; Microscopy, Electron/methods; Perfusion/instrumentation/methods; Reproducibility of Results; Tolonium Chloride
AD Biochemistry, Pathology and Parasitology Department, Central Veterinary Laboratory, New Haw, Addlestone, Surrey.
SP englisch
PO England