NR ALAI
AU Somerville,R.A.; Birkett,C.R.; Farquhar,C.F.; Hunter,N.; Goldmann,W.; Dornan,J.; Grover,D.; Hennion,R.M.; Percy,C.; Foster,J.; Jeffrey,M.J.
TI Immunodetection of PrPsc in spleens of some scrapie-infected sheep but not BSE-infected cows
QU Journal of General Virology 1997 Sep; 78(9): 2389-96
PT journal article
AB The development of diagnostic tools for transmissible spongiform encephalopathies (TSEs) would greatly assist their study and may provide assistance in controlling the disease. The detection of an abnormal form of the host protein PrP in noncentral nervous system tissues may form the basis for diagnosis of TSEs. Using a new antibody reagent to PrP produced in chickens, PrP can be readily detected in crude tissue extracts. PrP from uninfected spleen had a lower molecular mass range than PrP from brain, suggesting a lower degree of glycosylation. A simple method for detecting the abnormal form of the protein, PrPsc, in ruminant brain and spleen has been developed. PrPsc was detected in sheep spleen extracts from a flock affected by natural scrapie and was also found in spleens from some, but not all, experimental TSE cases. In spleens from cattle with bovine spongiform encephalopathy (BSE) no PrPsc was detected. It is therefore suggested that there is differential targeting of PrPsc deposition between organs in these different types of TSE infection which, with other factors, depends on strain of infecting agent.
ZR 39 Zitate
IN Immunologisch wurde proteaseresistentes Prionprotein in Milzgewebe von Schafen mit natürlich erworbenem Scrapie und einigen, aber nicht allen experimentell infizierten Schafen und nicht bei BSE-Rindern nachgewiesen.
MH Animal; Antibodies; Antibody Specificity; Blotting, Western/*methods; Brain Chemistry; Cattle; Chickens; Encephalopathy, Bovine Spongiform/metabolism; Female; Organ Specificity; PrPc Proteins/analysis/genetics/immunology; PrPsc Proteins/*analysis/isolation & purification; Recombinant Fusion Proteins; Scrapie/diagnosis/*metabolism; Sheep; Spleen/*chemistry; Support, Non-U.S. Gov't
AD Robert A. Somerville (robert.somerville@bbsrc.ac.uk), Christine F. Farquhar, Nora Hunter, Wilfred Goldmann, Jaqueline Dornan, James Foster, BBSRC & MRC Neuropathogenesis Unit, Institute for Animal Health, West Mains Road, Edinburgh EH9 3JF, UK; Christopher R. Birkett, Diana Grover, Ruth M. Hennion, Claire Percy, Institute for Animal Health, Compton, Nr Newbury, Berks RG20 7NN, UK; Martin J. Jeffrey, Lasswade Veterinary Laboratory, Bush Estate, Penicuik, Midlothian EH26 0SA, UK
SP englisch
PO England