NR ALAN
AU Somerville,R.A.; Merz,P.A.; Carp,R.I.
TI Partial copurification of scrapie-associated fibrils and scrapie infectivity
QU Intervirology 1986; 25(1): 48-55
PT journal article
AB The association between scrapie infectivity and scrapie-associated fibrils (SAF) during a partial purification procedure for infectivity was investigated. Scrapie infectivity and SAF can be separated from most membrane components by subcellular fractionation of infected mouse brain to obtain a synaptosomal fraction, followed by detergent treatment and density gradient centrifugation. After different detergent treatments, with either octyl glucoside or sodium N-lauroyl sarcosinate, SAF showed differing sedimentation characteristics but nevertheless cosedimented with scrapie infectivity in both cases. Copurification under two different conditions provides more evidence that SAF may be a form of the infectious agent of scrapie.
MH Animal; Brain/microbiology; Brain Chemistry; Detergents/pharmacology; Glucosides/pharmacology; Mice; Mice, Inbred C57BL; Molecular Weight; Nerve Tissue Proteins/biosynthesis/*isolation & purification; PrP 27-30 Protein; Prions/*pathogenicity; Sarcosine/analogs & derivatives/pharmacology; Synaptic Membranes/analysis; Synaptosomes/analysis/drug effects/microbiology
SP englisch
PO Schweiz