NR ALAQ
AU Somerville,R.A.; Millson,G.C.; Kimberlin,R.H.
TI Sensitivity of scrapie infectivity to detergents and 2-mercaptoethanol
QU Intervirology 1980; 13(2): 126-9
PT journal article
AB Exposure of fractions obtained from scrapie-infected mouse brain to Triton X-100 resulted in no change in its infectivity except after sedimentation. Infectivity was reduced after exposure to 2-mercaptoethanol but only in the presence of SDS. These results support the view that protein is an important component of the scrapie agent and that disulfide bonds may play a part in maintaining the structural integrity of the infectious agent.
IN Eine Behandlung mit Triton X-100 reduzierte die Infektiosität von Extrakten aus Scrapie-Hirnen nicht. 2-Mercaptoethanol reduzierte die Infektivität nur in Anwesenheit von SDS. Dies soll für die Notwendigkeit von Disulphidbindungen sprechen.
MH Animal; Brain/microbiology; Centrifugation; Chemistry; Lysophosphatidylcholines/pharmacology; Mercaptoethanol/*pharmacology; Mice; Polyethylene Glycols/*pharmacology; Prions/*growth & development; Scrapie/microbiology; Sheep; Sodium Dodecyl Sulfate/*pharmacology
SP englisch
PO Schweiz