NR ALGC
AU Sugaya,M.; Nakamura,K.; Watanabe,T.; Asahina,A.; Yasaka,N.; Koyama,Y.; Kusubata,M.; Ushiki,Y.K.; Kimura,K.M.; Morooka,A.; Irie,S.; Yokoyama,T.; Inoue,K.; Itohara,S.; Tamaki,K.
TI Expression of cellular prion-related protein by murine Langerhans cells and keratinocytes
QU Journal of Dermatological Science 2002 Feb; 28(2): 126-34
PT journal article
AB Transmissible spongiform encephalopathies are characterized by the accumulation of a proteinase-resistant isoform of the cellular prion-related protein (PrPc) within the central nervous system (CNS). The accumulation of scrapie-associated PrP (PrPsc) within cells of the lymphoreticular system prior to its accumulation in the CNS is regarded as important for the development of neurological diseases after peripheral inoculation. Little, however, is known as to which cells are the targets for peripheral inoculation. Here, the presence of PrPc on murine Langerhans cells (LC), dendritic cells in the skin and mucosa, and keratinocytes (KC) is demonstrated by immunohistochemical staining, Western-blotting and FACS analysis. The expression of PrPc mRNA in freshly purified LC and KC was also detected by reverse transcriptase-polymerase chain reaction. The expression of PrPc on LC was slightly increased during culture. These data suggest that LC and KC may be the targets for peripheral infection with prions.
MH Animal; Base Sequence; Female; Gene Expression; Immunohistochemistry; Keratinocytes/*metabolism; Langerhans Cells/*metabolism; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Knockout; Phosphoprotein Phosphatase/genetics/*metabolism; Prion Diseases/genetics/metabolism; RNA, Messenger/genetics/metabolism
AD Faculty of Medicine, Department of Dermatology, University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, 113-8655, Tokyo, Japan. sugayam-der@h.u-tokyo.ac.jp
SP englisch
PO Irland