NR ALGN
AU Sun,X.; Dong,X.; Zhang,B.
TI [Analysis and applications of a PrP antibody elicited by synthesized human PrP peptides]
QU Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese Journal of Experimental and Clinical Virology 2001 Dec; 15(4): 305-8
PT journal article
AB BACKGROUND: To further study the immuno-reactivity and specificity of a PrP antibody elicited by synthesized human PrP peptides. METHODS: Construction of a plasmid which expresses a N-terminus deleted human PrP protein and purification of this truncated protein. The prepared PrP antibody was tested for reaction with various prokaryotic expressed human PrP proteins,including the full-length protein,the N-terminus-and C-terminus-truncated proteins with Western blot. The antibody was also used in the immunofluorescence assays to identify the PrP proteins expressed from the cell lines transfected with human PrP genes. Moreover,the brain tissues from scrapie agents-infected hamsters and a CJD patient were extracted and the proteinase K-resistant PrPres were analyzed with the prepared antibody. RESULTS: The PrP-peptide elicited antibody could recognize both prokaryotic and eukaryotic expressed human PrP proteins, as well as the PrPres proteins in the brain tissues both from the scrapie-infected hamsters and CJD patient. CONCLUSIONS: The tested antibody possesses eligible immuno-reactivity and specificity and can be used for diagnosis of CJD in the clinical work.
MH Animal; Antibodies/*immunology; Antibody Specificity/immunology; CHO Cells; Creutzfeldt-Jakob Syndrome/diagnosis; English Abstract; Hamsters; Human; Peptide Fragments/*analysis; PrP 27-30 Protein/*immunology; Protein Precursors/*analysis; Scrapie/diagnosis; Support, Non-U.S. Gov't
AD Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
SP chinesisch
PO China