NR ALME
AU Tatzelt,J.; Groth,D.F.; Torchia,M.; Prusiner,S.B.; DeArmond,S.J.
TI Kinetics of prion protein accumulation in the CNS of mice with experimental scrapie
QU Journal of Neuropathology and Experimental Neurology 1999 Dec; 58(12): 1244-9
PT journal article
AB The kinetics of PrPsc and insoluble PrP accumulation in the spleens and brains of CD-1 mice were studied. The mice were inoculated intracerebrally with RML prions and euthanized at various times between inoculation and the onset of illness at approximately 130 days. Protease-resistant PrPsc, PrP 27-30, was first detected in brain by histoblotting 49 days after inoculation and by Western immunoblotting at 70 days. In spleen, PrP 27-30 was first detected by Western immunoblotting at 28 days after inoculation. Like PrP 27-30, substantial increases in detergent-insoluble PrP were first detected at 70 days after inoculation in brain and 28 days in spleen. In addition, a progressive increase in detergent-soluble PrP was detected beginning 70 days after inoculation. Further characterization of detergent soluble and insoluble PrP with respect to protease-sensitive PrPsc and prion infectivity will be of considerable interest.
MH Animal; Blotting, Western; Brain/*metabolism; Detergents/pharmacology; Kinetics; Mice; PrP 27-30 Protein/metabolism; PrPsc Proteins/metabolism; Prions/*metabolism; Scrapie/*metabolism; Solubility; Spleen/metabolism; Time Factors; Tissue Distribution
AD Department of Neurology, University of California, San Francisco 94143-0518, USA
SP englisch
PO USA