NR ALNC
AU Taylor,D.M.; Fernie,K.; McConnell,I.
TI Inactivation of the 22A strain of scrapie agent by autoclaving in sodium hydroxide
QU Veterinary Microbiology 1997 Nov; 58(2-4): 87-91
PT journal article
AB Samples of macerated mouse-brain infected with the 22A strain of scrapie agent were subjected to gravity-displacement autoclaving at 121 degrees C for 30 minutes in the presence of 2 M sodium hydroxide. No infectivity was detectable by mouse bioassay in samples which were either held for an hour at room temperature before autoclaving, or autoclaved immediately after adding the hydroxide. In contrast, all of the mice injected with a control sample, held for an hour in distilled water before autoclaving, developed scrapie. If adopted as a routine decontamination procedure it is recommended that hydroxide-treated waste should be autoclaved in sealed heat-resistant containers which can withstand the pressures involved. This would make the procedure safer for the operator, and avoid autoclave damage through exposure to sodium hydroxide.
IN Während alle Mäuse an Scrapie vom Stamm 22A starben, wenn das infektiöse Gehirnmaterial vor der intrazerebralen Injektion 30 Minuten bei 121° autoklaviert worden war, überlebten alle Mäuse nach der intrazerebralen Inokulation von genauso lange und heiß autoklaviertem infektiösem Hirngewebe, wenn dieses vor dem Autoklavieren mit 2 M Natronlauge gemischt worden war.
ZR 22
MH Animal; *Brain Chemistry; Heat; Mice; PrPsc Proteins/*chemistry; Scrapie/pathology; Sodium Hydroxide
AD BBSRC and MRC Neuropathogenesis Unit, Institute for Animal Health, Edinburgh, UK
SP englisch
PO Niederlande
OR Prion-Krankheiten 8