NR ALNG
AU Taylor,D.M.; McConnell,I.; Fraser,H.
TI Scrapie infection can be established readily through skin scarification in immunocompetent but not immunodeficient mice
QU Journal of General Virology 1996 Jul; 77(7): 1595-9
PT journal article
AB Scarification of the skin is a possible route of entry for scrapie infectivity in sheep, and for Creutzfeldt-Jakob disease agent in humans within the context of occupational exposure to infected brain in the autopsy room or laboratory. The effectiveness of skin scarification routes as portals of entry for infectivity had not previously been tested experimentally but this study has shown that these are efficient routes for establishing infection in mice using the 139A and ME7 strains of scrapie agent. Scarification had much the same efficiency as inoculation by the intraperitoneal, intravenous or perivenous routes but was not effective in immunocompromised (SCID) mice. It was concluded that replication of infectivity within the lymphoreticular system, which is precluded in SCID mice, is a necessary prerequisite for the development of infection in the central nervous system following inoculation via scarification.
IN Die Autoren konnten experimentell zeigen, dass Mäuse durch Anritzen der Haut mit den Scrapie-Erregerstämmen 139A und ME7 ähnlich effektiv wie durch Injektionen in die Bauchhöhle oder in bzw. neben Venen infiziert werden können. Die Infektion funktioniert allerdings nicht bei immunodefizienten SCID-Mäusen.
ZR 23
MH Animal; Brain/pathology; Immunocompetence/immunology; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, SCID; PrPsc Proteins/*immunology/isolation & purification; Scrapie/*immunology/transmission; Skin/*immunology
AD Institute for Animal Health, Edinburgh, UK
SP englisch
PO England
OR Prion-Krankheiten 8